recombinant human cxcl13  (R&D Systems)

Journal: iScience

Article Title: Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base

doi: 10.1016/j.isci.2024.108877

Figure Lengend Snippet:

Article Snippet: CXCL13 levels in NHP plasma were measured using Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit (bio-techne/R&D systems) following instruction, with Human CXCL13 protein at 2-fold dilutions start at 500pg/ml as the standard for quantification.

Techniques: Virus, Neutralization, Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: TNFR2 Signaling Enhances Suppressive Abilities of Human Circulating T Follicular Regulatory Cells.

doi: 10.4049/jimmunol.2100323

Figure Lengend Snippet: FIGURE 6. The migration ability of Tfr cells toward a CXCL13 gradient. The culture medium containing 03000 ng/ml recombinant human CXCL13 was placed in the lower chamber of the transwell. Tfr or Treg cells treated with MR2-1 or control for 4 d were placed in the upper chamber of the transwell. After 4 h of incubation, cells were collected from the lower chamber, and the number of collected cells was determined. (A and B) Migration index was cal- culated as follows: (number of cells migrated to lower chamber in the presence of CXCL13)/(number of migrated cells in the absence of CXCL13). (C and D) The percentage of cells that migrate to the lower chamber among the cells that were initially applied to the upper chamber is shown. Bars show mean ± SD of each group (n 5 3 per group). *p < 0.05 (two-tailed Student t test).

Article Snippet: In total, 50 ml of suspended cells was placed in the upper chamber of Transwell Polycarbonate Membrane Inserts (5-mm pore, 96 wells; Corning, Corning, NY), and the lower chambers were filled with 150 ml of RPMI-1640 medium containing 10% with recombinant human CXCL13 (R&D Systems).

Techniques: Migration, Recombinant, Control, Incubation, Two Tailed Test